University of Pécs , Hungary
Biological effects of electromagnetic waves: stimulation of cell proliferation and differentiation during regeneration as a unique property of THz impulses
Dr. Molnár is a senior research fellow at Ecophysiological and Environmental Toxicological Research Group, Balaton Limnological Research Institute of Eötvös Loránd Research Network, Tihany, Hungary and an associate professor at the Department of Comparative Anatomy and Developmental Biology, Institute of Biology Faculty of Natural Sciences, University of Pécs, Hungary. He is interested in the reparative regeneration of earthworms and its regulation with definite chemical and physical factors. He investigates the interaction of the immune and neural systems during regeneration and the effect of electromagnetic waves on tissue dedifferentiation and redifferentiation. He has been a Ph.D. supervisor of the Doctoral School of Biology and Sportbiology and the Doctoral School of Physics (University of Pécs). In the London Interdisciplinary Doctoral Programme (King’s College, London, UK) he was a co-supervisor of a specific regeneration topic of a Ph.D. dissertation (Brain regeneration, an earthworm phenomenon: from molecular characterization to bioengineering a functional “brain in a dish”).
Timing: 16:30:00 to 17:00:00 Paris Time
There is growing experimental evidence that all living things respond to electromagnetic waves. However, the published results, applying various experimental protocols and model animals, are controversial (both beneficial and disadvantageous effects of distinct electromagnetic irradiations have been reported). The exploration of the biological effects of any electromagnetic irradiation needs both an exact, reproducible optical background and a standardizable biological model. One of them (posterior segments regeneration of earthworms) has been developed in our laboratory. The kinetics of the segment renewing (with its biochemical, cytological, and histological backgrounds) has been elucidated under various physiological parameters. Therefore, its application in exact photobiological experiments can contribute to the recognition of the effects of defined electromagnetic irradiation on living things and supports the selection of relevant parameters for medical sciences. Earlier we have shown that single-cycle THz pulses of 5 µJ energy, 0.30 THz mean frequency, 293 kV/cm peak electric field, and 1 kHz repetition rate has been overridden the genetically determined, endogenously mediated segment renewing capacity of our model animal (Eisenia Andrei, Annelida, Oligochaeta, Lumbricidae). In contrast, to control specimens, a significantly higher number of renewing segments have been found in THz pulses exposed worms. The regenerated segments have had regular histological organization, no malformation has been seen in their tissues. In contrast, advanced tissue development has been recognized e.g. in body wall epithelium and muscles, circulatory system and immune cells, and especially in the renewing ventral nerve cord ganglia. No similar effects have been seen in green, or red light exposed experimental animals, and the possible heating effect of THz pulses has also been experimentally excluded. In this presentation we show the ultrastructural and some histochemical characteristics (collagen deposition in cicatrix, dedifferentiation and redifferentiation of muscle cells, development of neurotransmitter-specific cells, and iron distribution in old and new tissues) of distinct animal tissues exposed to defined THz pulses, suggesting their possible medical application.
Per A. Löthman
Kaiserslautern University of Applied Sciences, Germany
Cancer-derived Exosomes (CDE) and their Role in Nanomedicine
Dr. Per A. Löthman obtained his Ph.D. degree from Twente University, The Netherlands in the field of Magnetics and Self-assembly, and conducted research in Canada, France, and Germany on carbon nanotubes, Graphene, and related 2D nanomaterials. His research is interdisciplinary and involves sensors and sensing, 2D advanced materials, BioNanotechnology including DNA, S-layers, Viruses (archaea, bacteriophages), Biomolecular Architecture, Botany, and functional surfaces. Dr. Löthman has published over 80 scientifical articles, and several book chapters and serves as a reviewer and he is on the editorial board for several journals such as Nature, Nature Materials, Journal of Bioanalytical and Analytical Chemistry, Journal of Colloid and Interface Science, Thin Solid Films, Sensors and Actuators, Microsystems Technologies, Biophysical Reviews and Letters and International ??urnal of Applied Mathematics and Theoretical Physics (IJAMTP). Dr. Löthman is CSO (Chief Scientifical Officer) at Foviatech GmbH in Hamburg, Germany, a young innovative high-tech company in the field of advanced materials and artificial intelligence, and a senior lecturer in “Nanomedicine, Nanopharmacy” and “Sensors and Sensing in Engineering, Biology, and Medicine” (Kaiserslautern University) and “Mechatronics Systems and Design” (Hamburg University), Germany and “Interdisciplinary Manufacturing Engineering” (HTW Berlin) Germany.
Exosomes are extracellular vesicles (Evs) secreted by most eukaryotic cells and participate in intercellular communication. They are present in all body fluids and they are produced actively in tumor cells, which are released and utilized to facilitate tumor growth. Exosomes carry the components proteins, DNA, mRNA, microRNA, long noncoding RNA, circular RNA, etc., which play an important role in regulating tumor growth, metastasis, and angiogenesis in the process of cancer development, and can be used as a prognostic marker and/or grading basis for tumor patients, as therapeutic targets or even as anticancer drug carrier. Their characteristics enable them to assist major cancer hallmarks of cancer growth and spread. Cancer-derived exosomal trafficking is observed in several types of liquid or solid tumors, confirming their role as cancer hallmarks. In this contribution, we take a closer look at the roles of cancer-derived exosomes, of roughly 30– 100 nm diameter, in deregulating paracrine trafficking in the tumor microenvironment and circulation. Thus, exosomes are being exploited in diagnostic biomarker development, with their potential in clinical applications as therapeutic targets utilized in exosome-based nanoparticle drug delivery strategies for cancer therapy
University of Arizona, USA
Chemical doping and stability in organic semiconductors : A tale of two charge transfer states
Bharati Neelamraju is currently a process engineer at Applied Materials working on a carbon-based PECVD process for the next generation of memory applications. She has previously worked at Micron Technologies Inc as a postdoc in their pathfinding team for high aspect ratio cryo etch processes. Her background is in physics with a Ph.D. in materials science and engineering from the University of Arizona
Timing: 15:20:00 to 15:50:00 Paris Time
Organic semiconductors (OSCs) have incredible prospects for next-generation, flexible electronic devices including bioelectronics, optoelectronics, energy harvesting, and storage. They are flexible, biocompatible, printable, and low-cost semiconductor materials with control over chemical tolerability which gives us control over device properties. These properties expand the functionality of electronics beyond the current era of silicon making devices such as solar windows, skin patch sensors, wearable electronics, and foldable displays a possibility. However, they have lower conductivities compared to their inorganic counterparts, have a complex microstructure, and are prone to degradation. Hence, to make better performing systems we need to better understand the underlying mechanisms of doping and degradation in these systems and their intimate connection to microstructure and charge transport. We use a dopant F4TCNQ to p-type dope P3HT in the chemical doping
mechanism. It has been shown in the literature that this system either forms an integral charge transfer state (ICT) giving free charge carriers or a partial charge transfer state (CPX) forming traps based on processing techniques, with ICT being desirable. However, using a combination of spectroscopy, x-ray scattering, and conductivity measurements we show that these states exist simultaneously and that their existence is co-related to the local density of states of the semiconductor matrix. Using these new insights into their doping mechanisms, we turn to then evaluate the stability of the doped P3HT-F4TCNQ system in terms of its thermal degradation mechanism in the presence of varying environmental conditions.
University of Barcelona, Spain
Sensors integrated in Organ on chip platforms
Dr. Mònica Mir received a Degree in Chemistry from the University of Rovira I Virgili, Spain in 1998. In 2006 she received her Ph.D. in biotechnology at the same University. She realized different predoctoral stages at the Institute of Microelectronic in Demokritos, the University of Bath, and the National Hellenic Research Foundation. From 2007, she held a postdoctoral position at Max Planck Institute for Polymer Research, Germany. In 2008, she joins the Institute for Bioengineering of Catalonia (IBEC), Spain as a Senior CIBER researcher, combined with her teaching as an associate professor at the University of Barcelona. Throughout her carrier, she was managing European, National, and industrial research projects supervised Ph.D. ad Master's students, and collaborated in congress organizations as a coordinator and scientific committee. Her main scientific interests are focused on electrochemical biosensors, integrated into lab-on-a-chip and point-of-care technologies, implantable sensors, and organ-on-a-chip for biomedical applications.
Timing: 13:45:00 to 14:15:00 Paris Time
Nanotechnology is a cutting-edge field that spans many possibilities for the study and treatment of different diseases. A key tool recently developed in biomedical engineering research thanks to this technology is organ-on-chip (OoC) platforms. OoC is a low-cost and easy-to-work animal-free in vitro model that simulates the physiological response, considering mechanics and activity, of entire organ systems or part of them, for the study of human physiology (healthy and diseased) in a specific context of the organ. These platforms integrate nano and microfabrication techniques, microfluidics, multicellular 3D tissue, and monitoring integrated systems. Moreover, these micro-physiological systems permit the use of patient-induced cells, using human Induced Pluripotent Stem Cells (UPSC) will be a key tool for personalized pathophysiological study in vitro, and for permeability assessments of drug delivery for tailoring drug selection and dose, I mimicked body barriers. In this talk, an overview of OoC technologies that integrates sensor systems for continuous monitoring of the evolution of the micro-physiological platform will be presented. Future trends and advantages and limitations of this technology will be discussed.
Aristotle University of Thessaloniki (AUTH), Greece
Study of a new Anthracene-based polymer: From thin film characterization to fabrication of flexible yellow OLED devices
Mr. Kyparisis Papadopoulos holds a BSc in Physics and an MSc in Nanosciences& Nanotechnology from the Aristotle University of Thessaloniki. Currently, he is a Ph.D. candidate in Physics, also at the Aristotle University of Thessaloniki. He is specialized in Sheet-to-Sheet and Roll-to-Roll printing processes of organic electronics for techniques such as slot-die coating, screen printing, and spin coating. His work is focused on the field of Printed Organic Light-Emitting Diodes (OLEDs). Also, Mr. Papadopoulos participates in a national R&I project about developing a methodology and printing processes for large-scale OLED devices (project code: T1EDK-01039).
Timing: 10:45:00 to 11:10:00 Paris Time
Great efforts in both academia and industry have been made toward the development of bendable lighting, such as light-emitting devices on substrates that can be flexed and stretched. Organic Light-Emitting Diodes (OLEDs) are the perfect candidates for such applications. Design and synthesis of advanced polymeric emitting materials with appropriate electrical and optical properties are necessary for the realization of stable-performance OLEDs. Especially, yellow emissions can be applied to signaling applications, vehicle electronics, wearable electronics, etc. In this study, we focused on the characterization of a lab-scale polymer bearing bis(styryl)-anthracene moiety in terms of film-forming ability, emission characteristics, and color purity. The optical and photophysical properties of the solution-processable thin films were thoroughly studied via NIR-Vis-far UV Spectroscopic Ellipsometry (SE) and Photoluminescence (PL) respectively, whereas the structural characteristics were examined by Atomic Force Microscopy (AFM). Subsequently, yellow light OLED devices are fabricated by spin coating technique and characterized in terms of their electroluminescence properties and electrical characteristics. Finally, promising preliminary results on flexible OLEDs, using slot die coating processes, are reported.
Riga Technical University, Latvia
Morphology and Antibacterial Properties of ZnO-TiO2-SiO2 Sol-Gel Derived Coatings on Stainless Steel
Prof.Gundars Mezinskis is Dr.Sc.Eng. (1981) and Dr.HabilSc.Eng. (1998) in Faculty of Materials Science & Applied Chemistry of Riga Technical University (RTU). Leader of the Sol-Gel derived materials group of the Institute of Materials and Surface Engineering (RTU). He has authored over 110 publications (60 ISI publications among them) and author of 14 patents (2 EU patents among them). He led or participated in projects funded by national and European agencies as well as industrial companies. His research over the past 10 years has focused mainly on the development and study of coatings finding synthesis methods for material structure and material properties correlations.
Timing: 10:20:00 to 10:45:00 Paris Time
Biofilms are formed in different environments, on virtually any surface with sufficient moisture and nutrients, as well as hospital equipment surfaces resulting in causing biological contamination. The specific negative effects of bacteria biological pollution thanks to the growth of various bacterial resistance to antibacterial drugs is becoming a growing problem in medicine. It is concluded that antimicrobial resistance (AMR) poses a major threat to human health around the world .
We report the influence of ZnO-SiO2-TiO2 sol-gel dip-coating (single and multilayer) deposited on mirror-polished stainless steel AISI 304 (SS) modified by a phosphate conversion coating. Different parameters such as particle size of sols used, coatings mechanical durability, wettability, topography, and morphology (AFM, SEM/EDS) were analyzed (Fig.1). The antibacterial effect was determined in samples with a novel dried droplet method  using E. coli ATCC 25922 and S. aureus ATCC 6538 strains.
Results obtained show that phosphate SS and then sol-gel coated materials show a higher antibacterial effect than polished materials respectively 73,0% vs 21,3% in the case of S.aureus and 77,3% vs 44,3% in the case of E.coli (Figure 1). PhosphatedSS materials showed an antibacterial effect of more than 99% against E. coli and more than 95% against S.aureus.
Aristotle University of Thessaloniki (AUTH), Greece
Photophysical and Electroluminescence characterization of blue emitting polymers for OLEDs
Tselekidou Despoinaholds has a BSc in Physics from the University of Ioannina and an MSc in Nanotechnology from the Aristotle University of Thessaloniki (AUTH). Currently, she is a Ph.D. student at the Nanotechnology Lab LTFN, at AUTH. Her research interests involve the fabrication of thin films based on solution processes and the characterization through Spectroscopic Ellipsometry, Photoluminescence, Electroluminescence, and Atomic Force Microscopy. Specifically, her research activities focus on studying the properties of fluorescent and phosphorescent emitters for application in polymer OLEDs. In parallel, she holds 3 publications in international scientific journals, and she has participated in several international conferences.
Timing: 11:10:00 to 11:35:00 Paris Time
Conjugated polymers have received increasing attention owing to their outstanding electrical and optical properties and for this reason, they are promising candidates to apply as an emissive layer in Organic light-emitting diodes (OLEDs). Recently, efficient, and stable blue-emitting materials have been one of the most important prerequisites to kick off the commercialization of OLEDs. Consequently, the development of novel, high-performance, and stable blue light-emitting materials with color purity is crucial. Specifically, the blue light-emitting materials remained a great challenge and triggered the research interest, in order to achieve stable and high-quality light. This is due to the intrinsic wide band gaps of blue emissive materials, which generate a high charge injection barrier and unbalanced injection and transportation of charges. In this study, we focus on the photophysical characterization of one novel, lab-scale blue emissive polymer based on Carbazole derivatives compared to the commercially supplied based on Polyfluorene derivatives. The comparative study of these materials helped to evaluate their properties, through the synergy of Spectroscopic Ellipsometry, Photoluminescence, and Atomic Force Microscopy techniques. Following, these blue-emitting polymers are used as an emissive layer in OLEDs. The electrical characteristics of fabricated OLED devices are investigated as well as the stability of the electroluminescence emission spectrum during the device operation. Finally, the determination of the optical properties in combination with the photo- and electro-emission characteristics provide us to evaluate the color stability of each material.
Fraunhofer Institute for Reliability and Microintegration, Germany
Prototype fabrication of sub-millimeter sized batteries based on silicon wafer technology batteries
Robert Hahn received his Master's and Ph.D. degrees in electrical engineering from the Technische Universität Dresden. He is head of the micro energy storage group of Fraunhofer IZM. He works for more than 20 years in the field of batteries and microelectronic packaging. He has taken over the coordination of several national and European research projects for the development of new batteries and integrated power supplies for microsystems, energy autarkic, and medical electronics. He is head of the Fraunhofer micro battery initiative MicroLIB. Dr. Hahn has filed 30 patents in the area of micro-energy systems and authored and co-authored more than 100 journal and conference publications as well as book chapters. He was the coordinator of the FP7 project MATFLEXEND for the development of Lithium-ion batteries based on nanofibers. Since 2018 he is in charge of the micro battery prototyping line at Fraunhofer IZM that is used for the fabrication of micro-batteries for industrial customers.
Timing: 9:55:00 to 10:20:00 Paris Time
Several emerging innovations in fields like the internet of things, medical/health, nano-robotics, and the military require extremely miniaturized batteries and special form factors. A technology was developed that combines advanced silicon wafer-level packaging and micro-print and deposition processes to fabricate thousands of small batteries in parallel on a substrate. While standard lithium-ion electrode materials are used adaptions were made for the electrolyte to reduce the vapor pressure. A key technology is wafer fabrication on glass carriers because the silicon walls of the battery housing are very thin. Deep reactive ion etching is used to fabricate the battery housing, as well as PECVD oxide/nitride deposition to isolate the active battery materials from the silicon walls. Special focus is on the low-temperature hermetic sealing of the batteries on wafer-level since battery degradation and increase of internal resistance start at temperatures above 90 °C. A combined process of polymer bonding of the battery lid and additional metallization was developed to achieve both: an assembly and packaging technology that can be performed simultaneously on thousands of micro-batteries on the substrate and a robust and tight encapsulation.
The attainable energy density as a function of active materials and battery size will be shown. At battery dimensions between 1x1 … 10x10 mm2 and thickness between 200 μm and 1 mm the overall energy density is ca. 300 Wh/l. All available electrodes for lithium-ion batteries like graphite and lithium titanate anodes and NCA; NCM and LFP cathodes were qualified for the micro battery fabrication. With help of various electrode combinations, cell voltages between 1.5 and 4.0 Volts can be realized. Carbon nanotubes are used to increase electrical conductivity and reduce the contact resistance between electrodes and current collectors. Finally, a process yield of the battery fabrication process will be discussed.
University of Belgrade, Serbia
Ferro electric properties of BiFeO3 ceramics with cation substitutions at Bi-site (La3+, Eu3+) and Fe-site (Nb5+, Zr4+)
Dr. Aleksandar Radojkovic is a senior research fellow at the Institute for Multidisciplinary Research, University of Belgrade. he as worked Senior Research Fellow at, Institute for Multidisciplinary Research in 2022. Research Fellow, Institute for Multidisciplinary Research in 2014. Research Assistant, Institute for Multidisciplinary Research in 2010.His Education Ph.D. in Materials science, Faculty of Technology and Metallurgy, University of Belgrade, Dissertation title: “Properties of yttria doped barium cerium oxide ceramics as an electrolyte for solid oxide fuel cells”.in 2014He Studied BSc in Norwegian language and Scandinavian literature, Faculty of Philology, the University of Belgrade in 2004He studied MSc in Inorganic Chemical Engineering, Faculty of Technology and Metallurgy, the University of Belgrade in 2003His research interest is Proton conductivity and electrical properties of BaCeO3-based materials and their application in solid oxide fuel cells; Synthesis and characterization of multiferroic BiFeO3, using doping and codoping as a strategy for tailoring its ferroelectric and magnetic properties; Development of hybrid composites based on biopolymers and metal oxides as environmentally safe pesticides.
Timing: 9:30:00 to 9:55:00 Paris Time
BiFeO3 is one of few multiferroic perovskites that exhibits both magnetic and ferroelectric properties at room temperature. However, it is also distinguished by high leakage current, low remnant electric and magnetic polarization, and high electric coercive field. These features keep it away from any practical use in electronics. Therefore, many attempts have been done to improve the properties of BiFeO3 by Bi- or Fe-site doping or by both. Previous investigations suggest that doping with Nbat Fe-site can positively affect the magnetic behavior of BiFeO3 and decrease the leakage current.
In this study, a variety of cation substitutions at Bi-site (La3+, Eu3+) and Fe-site (Nb5+, Zr4+) were examined with the aim to investigate their possible synergism and benefit for the ferroelectric properties. The role of the cations with higher valence is to suppress the formation of structural defects during syntheses, such as oxygen and bismuth vacancies. These defects are responsible for high leakage currents, and consequently, low breakdown voltages characteristic of the pure BiFeO3. On the other hand, rare-earth cations at the Bi-site usually enable densification of the ceramics in a wider range of temperatures, preventing bismuth loss and formation of defects and secondary phases during sintering. However, do pant concentrations above 10–15mol% may give rise to transition from polar, rhombohedral (R3c) to non-polar, orthorhombic (Pnma) symmetry.
The carefully selected compositions of doped BiFeO3 were synthesized by a simple hydro-evaporation method. The ceramics samples were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), and polarization techniques, including leakage current measurements. Although it was shown that the introduction of Nb5+or Zr4+decreased the leakage current, they surprisingly deteriorated the ferroelectric properties even at concentrations as low as 1 mol%. This effect was more pronounced for the samples containing Nb. On the contrary, both La3+ and Eu3+(incorporated at the Bi-site) improved the ferroelectric properties as their concentrations increased, whereby the La-doped samples exhibited higher remnant electric polarization at observed electric fields. The highest remnant electric polarization of31.9 µC/cm2at 150 kV/cm, was measured for Bi0.85La0.15Fe0.998Zr0.002O3, indicating the synergetic effect of La3+ and Zr4+, which is limited to very low Zr4+concentrations.
Vajresh Kumar N
Indian Institute of Science, India
Functionalization of Fiber Bragg Grating Using Different Coating Methods
Optical fiber gratings have gone through an improved growth of development in the current years in track with the observation of narrow-band reflection in the photosensitive core region of silica optical fiber which is Germanium or boron-doped. Fiber Bragg grating is a periodic modulation of the refractive index along with the core of a photosensitive fiber. The refractive index changes are made by exposing the fiber to the interference pattern of Ultraviolet light. FBGs are less sensitive to refractive index (RI) variations of the external medium as the fiber is covered by cladding, limiting the application of FBGs being used in biological and chemical sensing applications. The surface of the e-FBG is modified with functionalized coatings based on the target to detect the change in RI using different coating techniques and different nanomaterials such as AgNP, AuNP, etc., and is characterized. This sensing probe can be used in different biological sensing applications.
Keywords: Fiber Bragg grating, coating techniques, nanoparticles, functionalization,
Tarbiat Modares University, Iran
Oocyte Characterization Using Microfluidic Channel
Dr. Davood Fathi received a B.Sc. degree in the field of electronic engineering from Amirkabir University of Technology, Tehran, Iran, in 1990, and an M.Sc. degree in the field of biomedical engineering from Sharif University of Technology, Tehran, Iran, in 1994. After a couple of years working in the industry, he worked toward a Ph.D. degree between 2006-2009 in the field of nanotechnology with the Nanoelectronic Center of Excellence, Thin Film and Photonics Research Laboratory, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.
Dr. Fathi has joined in 2010 as a member of the faculty of the Department of Electrical and Computer Engineering, Tarbiat Modares University (TMU), Tehran, Iran.
He has been the reviewer for more than 20 international journals including IEEE, OSA, Elsevier, Springer, John Wiley, and so on.
His current research interests include Nanoelectronics; Nanotechnology; Nanophotonics and Optoelectronics; Nano-biotechnology; Nano-microfluidics; Biosensors; Solar Energy.
He is also the author or co-author of more than 85 scientific journal and conference papers in various fields of Nanoelectronics, Nanotechnology, and Photonics.
Dr. Fathi is currently an Associate Professor in his department.
He has supervised more than 40 M.Sc. students and 5 Ph.D. students. One Ph.D. student and 3 M.Sc. students are currently working on an interdisciplinary topic of oocyte evaluation based on the design and fabrication of nano-micro fluidic systems.
He has been selected as an invited or keynotes speaker for several international conferences such as CCET-2022, CCT-2022, Nanotechnology 2022, Microfluidics 2022, Material Chemistry 2022, and so on.
Oocyte development is largely dependent on its mechanical properties. Cell mechanics control the outcome of cell division. In recent years, various techniques such as micropipette aspiration (MPA) and optical tweezers are being used to measure the mechanical properties of membranes. The traditional MPA systems, however, have low throughputs and limited potential for automation and require bulky infrastructures. These methods have been used to investigate the mechanical characteristics of single cells including the elastic and viscoelastic properties, etc. In MPA methods, by applying a specific suction pressure, the cell aspirates into a micropipette. The amount of aspirated surface into the pipette is then recorded and measured using an electron microscope over time. During the aspiration, any leakage between the cell and the micropipette needle causes an error in the measurement of suction pressure. Due to the complexity of pressure measurement, the accuracy of the results largely depends on the skill of the user of the equipment. Furthermore, surface water evaporation and bubbles in the micropipette lead to errors in measuring the applied pressure. Although the aspiration force is applied to the entire oocyte volume, since the direction of the applied pressure is random, the average values for the measured mechanical characteristics of the entire cell are considered. On the other hand, these techniques are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation.
Microfluidic channels have been developed in the past two decades for various biomedical applications, due to advantages such as real-time analysis, system integration, transparency for direct optical access, labor, precise control, lower cost, and reduced space. Furthermore, microfluidic channels can be integrated with other techniques and can enhance traditional cell analysis methods. Biological cell analysis and characterization can be performed based on the shape, size, and deformability of single cells in microfluidic chips. Various methods have been applied for microfluidic single-cell trapping including hydrodynamic trapping, dielectrophoresis trapping, chemical trapping, gel trapping, magnetic trapping, acoustic trapping, laser trapping, etc.
In this lecture, I present a new method for designing and manufacturing microfluidic channels in order to measure and evaluate the mechanical properties of mouse oocytes. The proposed process has the advantages of high accuracy and fast and easy operation. In our proposed technique, the oocytes are trapped in a specific channel, as shown in Fig. 1.
Although the designed and manufactured structure has microfluidic dimensions, in the design and analysis of the structure, knowledge and software environment in the field of nanotechnology and nanoelectronics have been used.
University of Pisa, Italy
Are nanoplastics toxic to plants? Early evidence and concerns
Carmelina Spanò joins the University of Pisa as a researcher in the Department of Biology. She received a degree in Biological Sciences from the University of Pisa, cum laude, and the title of Ph.D. in Agricultural Biology. She has held many courses in plant physiology (Plant Biochemistry, Plant Biotechnology, Plant Physiology, Transgenesis in plants, and currently Strategies of resistance in plants). Her primary research interests have been in plant response to abiotic stressors in laboratory-controlled conditions, with a particular interest in studying oxidative stress and antioxidant response in plants under different physiological or stress conditions. In the last decade, her interest has turned to the study of the effects of nanomaterials on plants. In her free time, she practices tai chi and brisk walking, and reads many novels, especially detective stories; she is also fond of jazz music.
Timing: 14:55:00 to 15:20:00 Paris Time
Plastics are used in many industrial sectors and in several everyday life products. The extensive use and the poor attention to their disposal and recycling are causing the production of massive amounts of waste, with possible global contamination of environmental matrices. As known, plastics are not biodegradable and remain in the environment for long periods undergoing aging processing with fragmentation into smaller pieces, up to micro (less than 5 mm) and nano (less than 100 nm) particles dimension, the smaller fractions being of specific concern. The possible entry of these particles into the food chain through plants represents a problem to deal with to improve food safety for humans and animals. To investigate the interactions between these particles and plants, we used a multidisciplinary approach in which seeds of the model crops Allium cepa L. and Oryza sativa L. were treated with different concentrations of polystyrene nano plastics (PS-NPS), chosen as model particles, as polystyrene is among the six more used plastics, widely diffused in all the terrestrial environments including agro-ecosystems. Under short-term conditions, phytotoxicity, genotoxicity, and cytotoxicity effects were assessed during germination and the early stages of seedling growth. Thanks to our integrated approach it was possible to highlight that PS-NPS can impair seed germination and seedling growth. They can be taken up by the root and translocated to the above-ground part of seedlings, even entering inside cells, where their presence is associated with ultra structural damages and genotoxicity. A different concentration/localization of main oxidative stress markers has been recorded, along with the induction of an enzymatic antioxidant response. Our results suggest that damages detected could be due not only to changes in the production/diffusion of oxidative stress markers but also to a possible direct effect of PS-NPS, able to overcome plant cell barriers. Further studies under long-term conditions, with a particular focus on the edible parts of the plant, will be useful to better clarify the impact on the environment and health of nano plastics, but their ability, even in short-term experiments, to enter inside crop plants, and possibly into the food chain is a matter of considerable concern.
Muhammad Sultan Payzullakhanov
Tashkent stat technical university, Uzbekistan
The cobaltite of barium and strontium, synthesized in a solar furnace
He was born in 1962. In 1984 graduated from Tashkent State University. in 1998 he defended his Ph.D. thesis at the Institute of Nuclear Physics. in 2019 He defended his doctoral dissertation at the Institute of Physics and Technology. works at the Institute of Materials Science of the Academy of Sciences of the Republic of Uzbekistan as the head of the laboratory. area of interest - use of concentrated solar energy, materials science. Based on scientific research on the interactions of concentrated solar radiation with materials in solid and liquid states, he published more than 145 publications, including 7 patents. Under his supervision, 1 Philosophy Doctor of Technical Sciences thesis was defended.
Perovskite cobaltites of strontium SrCoO3−δ and barium BaCoO3-d have been studied. It is shown that the technological route, which includes melting a stoichiometric mixture of cobalt oxide with barium or strontium carbonates in a solar furnace, quenching the melt into water, grinding the casting and molding, followed by sintering at 11000C, makes it possible to obtain a material based on hexagonal barium and strontium cobaltites with a developed fine microstructure and semiconductor properties. the nature of the electrical conductivity.
Keywords. Barium cobaltites, strontium, solar furnace, melting, melting, hardening, sintering, ceramics.
Diyala University, Iraq
Promising laser devices for optical communication, reliability, high-speeds and stability
Salam Nazhan currently works at Diyala University as an Assistant Professor in the Department of Communication, college of engineering. He received the BS and MS degrees in physics, and electronics physics from Al Mustansiriya University, Baghdad, Iraq, in 1998 and 2005 respectively, and the Ph.D. degree in optoelectronics from Northumbria University, Newcastle, the United Kingdom in 2016. Prior to attending the Optical Communications Research Group at Northumbria University, he worked as a researcher with the optoelectronics group at Bangor University, Wales, UK, for around one year from 2011 to 2012. His research focuses on the characterization of lasers for free-space optical communications, particularly, vertical-cavity surface-emitting lasers (VCSELs) devices. He published many research papers in the top journals in the field and several conference papers. He also presented his research at a number of international conferences and events around the world.
Timing: 17:00:00 to 17:25:00 Paris Time
At the present time, optical communications (OC) either free space or fibers, are using laser beams as a carrier for transmission signals through communication channels. This field of study has become substantially interesting in research paper topics in the last few years. Therefore, the investigation of OC based on laser beams gives attention greater than ever before in the research field. Free space optical communication (FSO) is concerned with data transmission through the atmosphere from one point to another using visible or invisible light to get a network connection. Optical sources or laser sources are utilized in such communication systems, it’s the key components to enable high-speed data transmission, reliability, and stability of the OC system. Laser sources offer advantages in bandwidth and speed for FSO applications over the traditional systems of communications. Lately, the laser beam increases the wireless capacity of 5G services and beyond for future applications. Recently, underwater OC based on laser devices has also given a wide interest in applications. Normally, FSO communication uses the wavelength range from short to long wavelength, which is from 700 nm to 1600 nm, because the wave optical energy which travels through the atmosphere has comparable properties at the visible and near-IR wavelengths. However, shorter and longer wavelengths of laser beams are also being considered for specific applications. The wavelengths between 780-850 nm are the most popular and widely used due to readily available and inexpensive components, which have an attenuation of less than 0.2 dB/km. The semiconductor laser diode applications employ high pump sources, like edge emitting lasers (EELs), which are the dominant and traditional source. However, EELs are too costly, requiring optical fiber coupling, which results in additional power loss. Therefore, attention has focused on vertical cavity surface emitting lasers (VCSELs) devices with potentially low manufacturing costs for various applications including OC systems. VCSEL is a semiconductor laser, which has a resonant cavity that is vertically formed on the surfaces of the epitaxial layers. VCSELs gained a reputation as a superior technology for applications such as Gigabit Ethernet and intra-systems, FSO communications, optical fiber communications, and optical recording. Furthermore, VCSEL with external optical feedback has become a hot security topic due to its encryption capabilities and is extremely popular in wide applications in modern communication. These brief words give highlight the topic and contribute to developing knowledge in a field of study.
Ordu University Faculty of Medicine., Turkey
Fluorescent and Biocompatible Nitrogen and Sulfur Co-Doped Carbon Nanodots as an Ocular Fundus Angiography Imaging Agent
Burak Erdem works as an assistant professor in the Department of Ophthalmology at Ordu University Faculty of Medicine. After completing his assistant training in ophthalmology at Haydarpasa Numune Research and Training Hospital, he focused on laboratory studies as well as clinical studies on the retina and glaucoma. He carries out three projects with his teammates, especially in the field of nanomedicine in ophthalmology.
The fluorescence characteristics and the toxicities of carbon nanodots are directly related to their elemental compositions. In this study, sulfur and nitrogen co-doped carbon nanodots (S/N-CDs) with their high quantum yield and low toxicity were hydrothermally produced. S/N-CDs were shown to be non-toxic in toxicity studies on HUVEC and L929 cell lines. In our in vivo experiment, one of the Whister-Albino-type rats received peritoneal injections of S/N-CDs, while the other had a conventional sodium-fluorescein injection, and ocular fundus angiography images were acquired. S/N-CDs were approved in vivo as an imaging agent for ocular fundus angiography and to serve as an alternative material for commercial fluorescent materials with its 85.5% of quantum yield.
Paula Fonseca Antunes Vieira
Paraíba University, Brazil
Analysis of the diagnostic potential of nano sensors in Breast Cancer 3D tumor
Paula Vieira is a Bachelor of Biomedical Sciences (2020) from Vale do Paraíba University. She began her MSc studies in Biomedical Engineering at the Nanosensors lab in 2021. with a scholarship from Coordination for the Improvement of Higher Education Personnel, CAPES [grant number: 88887.649530/2021-00]. She has experience with the synthesis and functionalization of nanoprobes, photodynamic therapy, 2D and 3D cell culture, xenografts, photosensitizers, and nanocomposites. Her research interests include nanotechnology, 2D and 3D cell culture, nanoprobes, photodynamic therapy, and non-conventional diagnosis/treatment methods.
Timing: 12:10:00 to 12:25:00 Paris Time
Overexpression of the Epidermal Growth Factor Receptor (EGFR) has been associated with malignancies with a worse prognosis, and the EGF protein activates its signaling pathways, which regulate cellular functions. As a result, the EGFR receptor is being investigated for a wide range of tumor diagnostics, encouraging the development of novel methods to improve quality and efficiency. Nanomaterials can recognize cancer cells by targeting certain biochemical pathways, highlighting nanomedicine's potential. Three-dimensional (3D) cell culture has arisen as an alternative to in vivo experiments for the formation of a heterogeneous microenvironment and the representativeness of the cellular mechanisms present in malignancies. Cell-cell and cell-extracellular matrix interaction is enhanced in 3D cell culture, preserving tissue shape and structure. In this regard, among the several types of 3D environment development that are conceivable, micro molded agarose allows large-scale reproducibility. In this study, breast cancer spheroids were created on micro molded agarose by the MDA-MB-468 strain. By functionalizing the EGF protein and Chlorine e6 (Ce6) in gold nanoparticles, the nanosensors were synthesized and applied to the spheroids for the detection of EGFR. Iron Oxide Nanoparticles Stabilized by Sodium Citrate. In: Second International Conference of Nanoscience and Nanobiotechnology, 2021, Brazil. In Vitro Matt Assay of Magnetic Nanoparticles Negatively Charged by Surfactant. In: Second International Conference of Nanoscience and EGF protein is delivered to the EGFR receptor through active targeting, and Ce6 serves as a fluorescent flag molecule. The tumors were cultivated for 21 days after being cast in 2% agarose molds. Flow cytometry was used to detect the presence of fluorescence and the cell death pathways after the nanosensors were applied. Annexin V (AnnV) and propidium iodide (PI) staining were used to assess cell death pathways in viable (AnnV- PI-), apoptotic (AnnV+ PI-), and necrotic (AnnV+ PI+) cells. The characterization of this tissue reveals the presence of fluorescence and the absence of substantial apoptosis and necrosis deaths.
King’s College London , UK
Synthesis and analysis of InP-based core/shell quantum dots using phosphinecarboxamide as a novel phosphide precursor
Yi has been a Ph.D. candidate in the Department of Physics at King’s College London since 2018. Her research project focuses on the synthesis of novel non-toxic core-shell structured InZnP-based quantum dots (QDs), phase transfer using a new kind of phosphide precursor, and applications of QDs in bioimaging. Prior to King’s, she obtained her master’s degree at Beijing University of Chemical Technology (Beijing, China) in 2018, where her work focused on thermally reversible silicone rubber & carbon nanotubes composite based on the Diels-Alder reaction. She enjoys cycling and hiking with friends to explore some exciting places in her spare time.
Timing: 14:40:00 to 14:55:00 Paris Time
Quantum dots (QDs) are now the most widely used nanomaterial for optical imaging, with a broad range of colors commercially available. Indium phosphide (InP) based QDs are a prospective replacement to the II-VI family of nanoparticles (NPs), particularly the cadmium (Cd) based ones currently used in the industry. InP-based QDs maintain good photoluminescence while being non-toxic, unlocking QD’s potential for clinical uses. In the past few decades, the choice of phosphide precursors in the synthesis of InP-based QDs has been restricted to tris(trimethylsilyl) phosphine and tris(dimethylamino) phosphine. Still, both are air-sensitive and must be dissolved in stock solutions in prior. This research synthesized InZnP/ZnS core/shell QDs in a one-pot reaction, using a novel air-stable precursor – phosphine carboxamide – by our collaborator from the University of Oxford. The emission full width at half maximum (FWHM) was found between 60 and 80 nm, although no attempt was made to narrow this. From TEM images, the particle diameters measured were 3.0 ± 0.1nm for the core InZnP QDs and 3.9 ± 0.2 nm for the InZnP/ZnS core/shell QDs. Its size and emission wavelengths are tuneable by adjusting the ratio of precursors, and/or the reaction time. The quantum yields (QC) for InZnP/ZnS core/shell QDs were found to be 36.28% without any shell modification such as HF etching. By applying the polymer encapsulation nanocrystal phase transfer technique using poly(styrene-co-maleic anhydride) (PSMA, 1700 g/mol) and ethanolamine (EA), the amphiphilic polymer/QDs formed have QYs at 28.32%. It can now be dissolved in water, opening up possibilities for clinical applications.
Kelly Cristina Quintela Martins Bittencourt
UniversidadeNove de Julho, Brazil
Photobiomodulation treatment modulates inflammatory mediators in DSS- induced colitis in mice
Kelly Cristina Quintela Martins Bittencourt Master's student of the postgraduate program in Biophotonics applied to Health Sciences at Universidade Nove de Julho. Dentist surgeon and Nurse graduated from Universidade Nove de Julho, specialization in digital dentistry and qualification in laser therapy, specialization in periodontics, implantology, pediatric dentistry, public health, and intensive care. Experience in the hospital and outpatient area, and in public health, with activities developed in the intensive care unit, surgical center, ostomy units, cancer center, in hospital infection control, and hospital quality monitoring programs.Wide use of photodynamic therapy and photobiomodulation in dental treatments, such as endodontics, periodontics, peri-implantitis, and lesions of the oral cavity.
Timing: 17:45:00 to 18:00:00 Paris Time
Colitis is a debilitating illness characterized by severe inflammation of the gastrointestinal tract. Treatments currently available are expensive and palliative. We here investigated the role of Photobiomodulation (PBM) on dextran sodium sulfate (DSS)-induced colitis. Male C57BL6 wild-type (WT) mice were divided into four groups: Control, PBM, DSS, and DSS+PBM. DSS was added to the drinking water of mice at days 0, 2, and 4 and withdrawn at day 6. PBM treatment was performed daily for 90s from day 6 to 9 on the right and left sides of the ventral surface and beside the external anal region (Wavelength 660 nm, Potency 100 mW, Total Energy 15 J). Our studies evaluated the production of IL-1beta, TNF-alfa, IL-6, IL-10, IFN-gamma, and TGF-beta in the bowel tissue. PBM treatment reduced IL-1 beta and IL-6 while increasing INF-gamma and TGF-beta in the bowel tissue. Thus, PBM treatment modulates the bowel inflammatory response, constituting a potential tool for treating colitis.
Gislene Freitas Franco
University of Sao Paulo, Brazil
Home-based photo biomodulation in the rehabilitation of patient swith chronic physical impairments. A systematic review.
Gislene Freitas Franco Master's student in Biophotonics Applied to Health Sciences at Universidade Nove de Julho in progress (2021). Graduated in Nursing from Bandeirante University of São Paulo (2006), Post-Graduation at Faculdades Metropolitana's Unidas in U.T.I. adult (2017), Post-Graduation in Dermatology and Aesthetics from the Faculty of Technology of Valença (2019), member of the Brazilian Society of Dermatology Nursing. Courses extracurricular courses in Surgical Instrumentation, Facial and Body Aesthetics, Qualification in Lasertherapy (2019), Training in Wound Treatment with Therapy Photobiomodulation (2019), Wound Assessment and Treatment (2019), Update on Wound Care (2019), The importance of the Skin Specialist Nurse in the ICU (2019), I Journey of Stomatherapy. from Hospital Sírio Libanês (2019), Training in Epilation a Led (2020), participation and publication of an article in the Estetika Congress (2019) and at the 8th Brazilian Congress of Nursing in Dermatology (2020). Since 2016 inserted in the dermatological clinical context. Currently works at Instituto INA.
Timing: 18:00:00 to 18:15:00 Paris Time
Sensorimotor impairments reflect the main sequelae of chronic and/or degenerative disorders that affect the neuromuscular and musculoskeletal systems and that require intervention and specialized care in ways to limit disability, functional rehabilitation, and improve the quality of life of the patients. Many muscular and/or neurovascular diseases, such as osteoarthritis and fibromyalgia, lymphedema, or neurological sequelae, could produce chronic impairment mental/or disabilities. As a result, during long-term follow-up, intra-hospital rehabilitation treatment can determine low adherence, high costs, and mobility difficulty. Domestic treatment could reduce costs, improve satisfactory results, and improve the qualityof life of the population. It isespecially important because COVID-19 highlights the urgency of using the home environment for continuity of healthcare. Inthis sense, thisstudyis a PRISMA systematic review todiscussif home-based photobiomodulation is a safe and effective technique in the treatment of patients with chronic physical impairments. We are interested in evaluating the changes in symptoms, functionality, and quality of life of patients with chronic impairments using home rehabilitation with photobiomodulation therapies. Our protocol was registered on the prosperous website before data extraction by No. CDR42022326588 and followed the Prisma guidelines. Clinical trials, case reports, guidelines, and observational studies evaluating the use of PBM in patients with chronic impairments of any etiology will be analyzed and available data about the quality of life, functionality, or degree of independence will be reported. The chosen tools for quality analysis of the included studies depend on the design of the data source. We intend to use the Cochrane risk-of-bias tool (RoB 2) for randomized trials; Cochrane Robins-I for non-randomized trials; and the CARE Tool for case reports. In addition, we will use the WALT recommendations to analyze the PBM parameters. If a minimum of two studies demonstrate population, stimulation parameters, and outcomes homogeneity, also intend to carry out a quantitative analysis of the extracted results (meta-analysis).
Ione Liz Paiotti
University Nove de Julho, Brazil
Effects of photobiomodulation on the prevention of the skin pressure injury in patients with a diagnosis of COVID-19: a randomized, controlled, and double blind clinical study protocol.
Ione Liz Paiotti Graduated in Nursing and Obstetrics from de University of Taubaté (1981). Specialist in Hospital Administration at São Camilo, in Occupational Nursing by FUNDACENTRO, and Intensive Care at the University of Taubaté. I worked as a lecturer in a nursing technician course at Robert Wood Johnson Foundation School, in the chemical industry (Monsanto) as a Labor Nurse, and as a public servant in primary care. I implemented one of the first AMEs of the State of São Paulo, in the municipality of São José dos Campos. Worked in public and private hospitals as coordinator of Adult, Pediatric, and Neonatal ICU for 14 years. My greatest experience is in the area of hospital management. At the moment, I work as Director of Nursing in a public hospital, managed by SPDM (Paulista Association for the Development of Medicine), of university Nove de Julho and I am a master’s student in the Graduate Program in Biophotonics Applied to Health Science of the University Nove de Julho (UNINOVE), São Paulo, SP, Brazil.
Timing: 18:30:00 to 18:45:00 Paris Time
The high incidence of pressure injuries (PI) is considered a serious public health problem and a negative indicator of the quality of nursing care. Objective: This study aims to verify the preventive effects of the use of photobiomodulation (FBM) in areas more susceptible to the development of PI in patients hospitalized with COVID-19. Methods: This is a controlled, randomized, and blind clinical study including hospitalized participants with a risk of developing PI according to the Braden scale. Participants will be randomized into 2 groups: Group 1 - Control (n=70) in which the hospital's standard operating procedures for the prevention of PI will be performed; and Group 2 - FBM (n=70) the same procedures as the group control and also FBM will be performed once a day, for 10 minutes in each of the 3 regions most commonly affected by PI, that is, sacral and calcaneal (bilaterally). The FBM will be performed using a plate with 132 LEDs of 660nm and 132 LEDs of 850nm(each LED has P=8 mW; E=4.89J, radiant exposure= 9.6 J/cm2; irradiance 16 mW /cm², 10 min). The incidence of PI will be evaluated every 48 hours after hospital admission for a period of 1 month or until hospital discharge if it occurs before this period. The time of onset of PI will also be evaluated, and the possible correlations of anthropometric data measurements and incidence of PI. The data will be statistically evaluated.
Carolina Montovam Monteiro
Universidade Nove de Julho, Brazil
Nonionic and anionic polymers affect methylene blue aggregation in formulations for antimicrobial photodynamic therapy
Carolina Montovam Monteiro is a Ph.D. student in Biophotonics applied to Health Sciences, has a Master's degree in Pharmacy (area of bioactive natural and synthetic products), a specialist in Homeopathic Pharmacy, graduated in Pharmacy and Biochemistry and Biological Sciences. She has experience as a compounding pharmacist for 21 years and as a professor in the pharmaceutical area for 12 years. She has been working as an undergraduate and graduate professor and author of books and teaching materials. She is currently a dative defender for the Ethics Committee of the Regional Council of Pharmacy of the State of São Paulo (CRF-SP) and a pharmaceutical consultant in compounding pharmacies, working mainly with legislation and regulatory matters, technical-administrative management, quality assurance, pharmacy technical development of cosmetics, homeopathic and allopathic medicines intended for topical, systemic and dental purposes, for human and veterinary use.
Timing: 18:45:00 to 19:00:00 Paris Time
Methylene blue (MB) is a cationic phenothiazinium dye with interesting properties in antimicrobial photodynamic therapy (PDT). The aggregation state directs the mechanisms of action, which means that type I or II oxidative reactions can be favored according to the medium in which MB is conveyed (1,2). The purpose of this study is to develop MB formulations associated with polymers for aPDT, considering the optimization of rheological properties, and dimer-to-monomer ratio (D/M) and aims to bring improvements in clinical applicability. Methodology & Theoretical Orientation: Hydroxypropyl methylcellulose (HPMC) and Carboxymethyl cellulose (CMC) were used, respectively, as nonionic and anionic polymers, to reach a viscosity between 30 and 450 cP. The modulation of the MB aggregation state was evaluated in the presence or absence of the anionic surfactant sodium dodecyl sulfate (SDS). In triplicate, absorption spectra were recorded in a UV-Visible UV-1800 spectrophotometer (Shimadzu, Japan) from 250 to 800 nm using a 2 mm pathway cuvette. Absorption values determined the D/M at 614 nm (dimer) and 662 nm (monomer). The D/M values underwent a logarithmic transformation and were statistically analyzed using the one-way ANOVA test, followed by the Tukey post-test, adopting ?=0.05. Findings: There was a lower D/M ratio with an increasing concentration of polymers in the absence of SDS, however with the addition of surfactant, there was a D/M reduction in formulations containing both polymers studied, with lower MB aggregation being observed in the formulation containing the anionic polymer CMC when compared to the nonionic polymer HPMC. Conclusion & Significance: The addition of SDS to the medium in which the photosensitizer was conveyed and the ionic charge of the polymer, influenced the MB aggregation behavior. Further adjustments to this formulation will be necessary to control MB aggregation.
Priscilla Farias Chagas
Estado university Rio de janeiro, Brazil
ANALYSIS OF THE ACTION OF PHOTODYNAMIC THERAPY IN TISSUE REPAIR OF PEOPLE AFFECTED BY DIABETIC FOOT LESIONS
Timing: 19:00:00 to 19:15:00 Paris Time
Studies point to the positive response that photobiomodulation has in diabetic foot lesions. However, there is still a lack of cost analysis of this therapy, which has been growing more and more, but in the treatment of lesions. The aim of this study is to analyze the response of the action of photodynamic therapy in tissue repair of people affected by diabetic foot injuries and to identify the cost of this treatment. It will be a double blind, randomized study, patients will be divided into two groups, a control and an experiment. The research will be carried out in a Municipal Health Center (CMS) located in the west of the city of Rio de Janeiro, Brazil. As inclusion criteria, the following were listed: patients affected by neuropathic lesions of the diabetic foot, patients with good adherence to the therapeutic plan, assiduous patients in consultations, patients assisted by CAP 5.1. As an exclusion criterion, the following were listed: patients under 18 years of age; patients with wounds with etiologies that are not related to the diabetic foot, patients with ischemic diabetic foot who have an ankle-brachial index (ABI) with a value less than 0.9 or greater than 1.3. Patients with glycated hemoglobin greater than 8%. Data collection will be carried out through the medical records, in order to establish the sociodemographic and clinical profile of patients affected by diabetic foot injury and through the nursing consultation three times a week, where the patient will be submitted to the proposed treatment, being performed 10 sessions of photodynamic therapy. In all consultations, through a data collection instrument, such information will be collected to monitor the evolution of the lesions: lesion size, lesion characteristics (odor, type of exudate, type of tissue found in the wound bed, of the lesion, perilesional area and presence of biofilm and/or infection). The sample size will be 68 patients. This value was calculated to provide 95% power (α = 0.05). Initial descriptive analyzes will be performed considering all variables measured in the study, both quantitative (mean, median and standard deviation) and qualitative (frequency and percentage).
Ana Carolina Alves Camargo Tortamano
Universidade Nove de Julho, Brazil
Comparative Study of Antimicrobial Photodynamic Therapy (aPDT) with and without Sodium Dodecyl Sulfate (SDS) for Aggregatibacter actinomycetemcomitans Biofilm Inactivation
My name is Ana Carolina Alves Camargo Tortamano, I'm a dentist. I have a degree in Dentistry from Universidade Paulista (UNIP) in 2014, specialization in periodontics from Universidade Paulista (UNIP) in 2017, a master’s degree in Biophotonics applied to health sciences from Universidade Nove de Julho (UNINOVE) in 2019 and I am currently studying for a PhD in Biophotonics. applied to health sciences by the Universidade Nove de Julho. I am a professor of higher education at the Universidade Nove de Julho.
Timing: 19:15:00 to 19:30:00 Paris Time
Aggregatibacter actinomycetemcomitans is a facultative anaerobic Gram-negative bacterium that is associated with periodontal disease. As an adjunct intervention to mechanical periodontal treatment, antimicrobial photodynamic therapy (aPDT) aims to produce reactive oxygen species to decrease microbial infection. It has been shown that sodium dodecyl sulfate (SDS) can improve aPDT results by increasing the formation of methylene blue (MB) monomers. The aim of this study is to compare the behavior of aPDT mediated by methylene blue in phosphate buffered solution (PBS) and with sodium dodecyl sulfate (SDS) in bacterial killing of A. actinomycetemcomitans. The biofilm will be grown on bovine dental samples and aPDT will be performed on them. Microbiological evaluation, scanning electron microscopy, and verification of the formation of reactive oxygen species will be carried out.
For this, 2 groups will be created: PBS Group and SDS Group; and 6 subgroups: Control (not treated with laser or photosensitizer); FS (exposure to MB 100 mM photosensitizer for 1 min); Laser, irradiated for 5 min in the absence of FS; and three aPDT subgroups, with three exposure times of 1, 3 and 5 min of irradiation. A laser (Photon Lase III, DMC, São Carlos, Brazil) with a wavelength of 660 nm, output power of 0.1 W, well irradiation of 250 mW/cm2, energy of 6, 18 and 30 J will be used. corresponding to the time of 60, 180 and 300s and radiant exposure of 15, 45, 75 J/cm2. Subsequently, microaerophiles will be cultured, counted and converted into colony forming units per mL for analysis and comparison.
Sharif University of Technology, Iran
The investigation of photocatalytic properties ofNi0.8CO0.2/Zn0.4Cd0.6S/g-C3N4as a nano-photocatalystfor pollutants, degradation, andH2 generationbywater splitting
Fatemeh Sousani joined the department of material science and engineering of the Shariff University of Technology as a doctoral candidate in September 2020. Her doctoral thesis is entitled “the performance improvement of H2 production via water splitting with NixCo1-x/ZnxCd1−xS/g-C3N4 nano hybrid photocatalysts” under the supervision of Prof.SayedKhatiboleslam Sadrnezhaad and Dr. Parvin Abachi. She received her master’s degree in 2017. She worked on the design of the germanium–carbon antireflection coatings. She had also a research project to investigate the thermal stability properties of germanium-carbon coatings. The results of her master’s thesis were published in reputable ISI journals.
Timing: 12:25:00 to 12:45:00 Paris Time
Graphitic carbon nitride (g-C3N4) is a promising metal-free visible-light photocatalyst due to its strong visible light response, high thermal and chemical stability, and available low-cost raw materials. This substance has gained significant attention for the degradation of pollutants and photocatalytic water splitting for H2 production under visible light. Nevertheless, due to the relatively large band-gap, low charge-carrier mobility, and thus limited effective use of sunlight, the photocatalytic hydrogen evolution efficiency of pristine g-C3N4is still unfavorable. Several strategies, such as morphological control of g-C3N4, creating heterojunctions with semiconductors, and doping with other elements have improved its photocatalytic efficiency. This study's aim is to improve the photocatalytic performance of g-C3N4for degradation of pollutants and hydrogen evolution by creating Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4 nano-photocatalyst that makes it more effective under visible-light irradiation. Here, Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4nano-photocatalyst is produced via a facile hydrothermal and chemical reduction method. Then, the morphology, structural, optical, and photocatalytic properties of the nanohybrid were characterized by field emission scanning electron microscope (FE-SEM), X? ray diffraction (XRD), Brunauer–Emmett–Teller (BET), UV? vis diffuse reflectance absorption spectra (DRS) and photoluminescence (PL). With the formation of Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4, the bandgap energy of the g-C3N4 decreases from 3.01 to 2.77 eV, and the absorption edge increases from 412 to 448 nm. The Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4absorption edge is located between g-C3N4 and Zn0.4Cd0.6S and has a stronger visible light absorption compared to g-C3N4 and Ni0.8Co0.2/Zn0.4Cd0.6S in the range of 410 to 620 nm. The performance of the Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4nano-photocatalyst in the removal of methylene blue (MB)from an aqueous solution can be used as a basis for its proper performance for water splitting and H2 production too. Photocatalytic degradation of methylene blue in an aqueous solution was carried out under an LED lamp (100 W, 400-700 nm) illumination for 120 min. It was found that the degradation efficiency of methylene blue was 96% within this period. Thus, the Ni0.8Co0.2/Zn0.4Cd0.6S/g-C3N4nano-photocatalyst can act as a potential photocatalytic material for the degradation of pollutants and production of hydrogen under visible light.
University of Szczecin, Poland
Metabolic Insertion of Titanium Into the 3D Structure of Diatomaceous Biosilica
M.Sc. Weronika Brzozowska obtained MA degrees in Quantum Chemistry and Forensic Chemistry at the Faculty of Chemistry at the Nicolaus Copernicus University in Torun in 2016 and 2019. During her second master's thesis research, she implemented the Ministry of National Defense project entitled "Study of the physicochemical properties of intelligent composites with the use of electroactive materials" in cooperation with the Polish Naval Academy based in Gdynia. Currently, she works on modifying algae - diatoms -with metal ions to obtain innovative nano-silica materials with unique optoelectronic properties and semiconductors. These activities are directly related to the doctoral studies that she carries out as part of the Doctoral School of the University of Szczecin (US), in the discipline of Earth and Environmental Sciences. She is also a member of the US research team implementing the project "Advanced biocomposites for the economy of tomorrow BIO-GNET".
Timing: 12:45:00 to 13:05:00 Paris Time
In the development of modern technologies, microorganisms, particularly unicellular algae (microalgae), are the growing inspiration due to their abundance and unique properties . Microalgae have the capacity to synthesize phenomenal mineral composites under natural conditions (calcium carbonate, silica) with complex hierarchic structures. The intricately ornamented silica shells known as frustules are a unique feature of diatom cells. The siliceous walls of frustules are perforated by periodic arrays of pores with diameters ranging from nano- to microscale forming openwork three-dimensional (3D) silica structures. Obtaining such structures of inorganic materials is one of the main challenges in the new materials and devices development. According to published works, the diatomaceous biosilica characterized by perfectly ordered3-D structure, thermal and mechanical stability, unique optical properties, and biocompatibility can be a valuable resource for applications in modern technologies for the production of optoelectronic devices, biosensors, gas sensors, catalysts, adsorbents, efficient filters, semiconductors, solar cells, templates for nanolithography, drug carriers or building material in the synthesis of bone implants [4,5].
The main aim of this study was to evaluate the ability of the diatom species Pseudostaurosiratrainorii to metabolically insert soluble titanium from a culture medium into the structure of their amorphous silica cell walls, by the cultivation of selected diatom species under laboratory conditions. The culture of diatom species was cultivated using Erlenmeyer flasks (3000 ml) with Guillard’sF/2 growth medium with soluble titanium concentrations range by2.5 mg/l to 90 mg/l and soluble silicon concertations ranging from 1.2 mg/l to 500 mg/l.
The morphological and structural features, elemental composition, structure, photoluminescence properties, and thermal stability of the obtained biosilica were examined using a set of techniques comprising scanning electron microscopy, transmission electron microscopy, and photoluminescence spectroscopy, IR spectroscopy, X-ray powder diffraction, and thermogravimetry. The figure shows some of the absorbing and compelling results obtained during the doping of diatomaceous silica with titanium ions.
Vale do Paraiba University, Brazil
Theranosticcharacterization of breast cancer treated with NanoprobesandThermal Ablation Therapy
Marcela Cândido received a chemical engineering degree from Vale do Paraiba University in 2018. She has been a Ph.D. student at Nanosensors Laboratory (IP&D/UNIVAP), with a scholarship from São Paulo Research Foundation, FAPESP [grant number 2019/26353-3]. Her expertise is in the field of nanoparticle synthesis and functionalization, molecule modification as well as photodynamic and photothermic therapies in cell cultures.
Timing: 11:50:00 to 12:10:00 Paris Time
Cancer is a problem for public health and new types of therapies have been studied. Among them, Photothermal Therapy (PTT) has been investigated in recent years, primarily as a non-invasive cancer treatment with fewer side effects for patients. Heat energy is produced when iron or gold nanoparticles are irradiated in the near-infrared range (700-1000 nm), which is capable to start the death process for a specific cell because tumor cells have a lower heat tolerance than normal ones. SPIONs@Au are multifunctional core-shell nanoparticles with an iron core that improve the material's chemical, physical, and optical capabilities. In this context, we investigated the synthesis and characterization of Au@SPIONs, as well as the surface functionalization with EGF-α-lipoic acid (increasing specificity for cells with EGFR overexpression) and chlorin e6 (Ce6)-cysteamine complexes, composing a theranosticnanoprobe (TP). The Confocal Fluorescence Microscopy evaluated TP internalization in the triple-negative breast cancer (TNC) cell line, which has a poor prognosis. Also, PTT was performed associated with Au@SPIONs and TP. The effectiveness of the technique was determined by flow cytometry, labeling viable cells, apoptotic cells, and necrotic cells. The results, SPIONs@Au showed a color change to red, and the presence of an absorption band centered at 530 nm. The Ce6-cysteamine complex was formed efficiently, having a resonant band at 670 nm, allowing the diagnosis in biological samples via fluorescence. Internalization of the TP in the cell cytoplasm was confirmed, indicating that it might be used as a diagnostic marker. The PPT in TNC produced positive outcomes, with an apoptosis death preponderance. Aside from that, the functionalized ce6 complex permits Photodynamic Therapy to be used, broadening the variety of uses.
Goethe University, Frankfurt, Germany
Laser-induced thermotherapy as minimally invasive treatment for hepatocellular carcinoma
Hamzah Adwan is a medical doctor who graduated in 2021 from Goethe-University Frankfurt am Main, Germany, and is currently doing his residency in Radiology. He has been doing research since 2018 at the Department of Radiology at Goethe-University Frankfurt am Main in the field of Interventional Oncology under the supervision of Professor Thomas J. Vogl. He has presented his work at a number of conferences and society meetings such as the CIRSE, IMSCB, ECR, Deutscher Röntgenkongress, and IROS. In addition, he has won a number of awards and recognitions including second prize winner of the oral presentation at the clinical sciences section at IMSCB 2020, and Scholarship holder of ‚Die hellsten Köpfe für die Radiologie‘ (brightest minds of Radiology) at Deutscher Röntgenkongress 2019&2020. He was selected as the ambassador of IMSSB 2021. More recently, his abstract for the IROS 2022 in Salzburg/Austria was selected to be one of the ten best abstracts presented at the congress.
Timing: 17:25:00 to 17:45:00 Paris Time
Hepatocellular carcinoma (HCC) is the most common primary tumor of the liver and one of the most common malignancies worldwide. There are many treatment options for HCC depending on the tumor stage such as liver transplantation, surgical resection, and locoregional treatments. These locoregional treatments are varied and include Transarterial chemoembolization (TACE)as well as thermal ablation such as radiofrequency ablation (RFA), microwave ablation (MWA), and laser-induced thermotherapy (LITT).
LITT has unfortunately been less investigated compared to other thermoablative therapies. This makes the study unique, and interesting especially due to the many examined parameters.
The aim of this study was to retrospectively evaluate the MR-guided LITT in the treatment of HCC, according to treatment response, survival rates, and complications. This study enrolled 53 patients (12 women and 41 men; mean age: 67.5 ± 8 years) with 75 HCC lesions. All sessions were performed in analgosedation and as an outpatient procedure. The included cases were investigated the based on size of tumors, ablation time, technical success, size of the post-ablation area, complete ablation, complications, local tumor progression (LTP), intrahepatic distant recurrence (IDR), overall survival (OS), and disease-free survival (DFS). Therapy response was assessed using contrast-enhanced MRI.
A total of 76 LITT sessions were performed. The mean pre-ablation axial diameter of the tumor was 2.4 ± 0.9 cm. Technical success was achieved in all sessions. The mean diameter of the ablation area was 5.3± 1.8 cm. The mean ablation time was 16.7±7.4 min. Complete ablation was achieved in 98.7% (74/75) of the treated tumors. The rates of LTP and IDR were 3.8% and 64.2%, respectively. The 1-, 3-, and 5-year OS rates were 96.2%, 54.7%, and 30.2%, respectively. The 1-, 2-, and 3-year DFS rates were 54.7%, 30.2%, and 17%, respectively. There were no treatment-related deaths or major complications among the patients. The rate of minor complications was 7.9% (6/76). LITT is safe and effective as a local treatment for HCC, which can be performed in a short time, and as an outpatient procedure without the requirement of general anesthesia. HCC patients treated by LITT had a high OS time and low rate of LTP.